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Related Experiment Videos

A flow system for partial automation of plating efficiency tests

S Macevicz, P Dean, D Peters

    Cytometry
    |March 1, 1981
    PubMed
    Summary
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    A new automated cell plating system sterilely deposits cells, reducing labor in tissue culture. While efficient for KHT and CHO-AA8 cells, it decreased plating efficiency for CHO-T5 cells.

    Area of Science:

    • Biotechnology
    • Cell Biology
    • Laboratory Automation

    Background:

    • Standard cell plating procedures in tissue culture are labor-intensive and involve manual dilution steps.
    • Automating cell deposition can potentially streamline workflows and improve consistency.

    Purpose of the Study:

    • To construct and evaluate a novel flow system for sterilely depositing a known number of cells into a collection vessel.
    • To assess the impact of automated cell plating on labor and the biologic effect on different cell lines.

    Main Methods:

    • A flow system was developed utilizing a nozzle to center single cells in a jet.
    • Cells were detected, sized, and counted using laser-induced light scattering.
    • Plating efficiency tests were performed comparing the automated system with manual plating for KHT, CHO-T5, and CHO-AA8 cells.

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    Main Results:

    • The automated system significantly reduced labor by eliminating the manual dilution step.
    • Plating efficiencies for KHT and CHO-AA8 cells were unaffected by machine plating compared to hand plating.
    • A reduction in plating efficiency was observed for CHO-T5 cells when using the machine plating system.

    Conclusions:

    • The developed flow system offers a labor-saving alternative for cell plating in tissue culture.
    • The biologic effect of machine plating is cell-line dependent, with CHO-T5 cells showing reduced plating efficiency.
    • Further optimization may be needed for specific cell lines to ensure consistent plating efficiency.