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Related Experiment Videos

Morphometry of nuclear pore complexes in thyroid cells during hyperplasia and involution

M C Many, J F Denef, A C Cordier

    Molecular and Cellular Endocrinology
    |August 1, 1981
    PubMed
    Summary

    Thyroid stimulation increases nuclear pore complexes and their density, correlating with cell activity, not the cell cycle. Involution normalizes these nuclear parameters.

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    Area of Science:

    • Cell Biology
    • Molecular Biology
    • Biophysics

    Background:

    • Nuclear pore complexes (NPCs) regulate transport between the nucleus and cytoplasm.
    • NPCs are crucial for cellular function and are found in the nuclear envelope.
    • Their regulation and distribution are key to understanding nuclear transport dynamics.

    Purpose of the Study:

    • To investigate the dynamic changes in nuclear pore complexes during thyroid stimulation and involution.
    • To determine the relationship between NPC number, numerical density, and cellular activity.
    • To elucidate the mechanisms of NPC biogenesis and degradation in thyroid follicular cells.

    Main Methods:

    • Analysis of nuclear pore complexes using freeze-fractured replicas of C3H mouse thyroid follicular cells.

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  • Examination of cells in various physiological states, including thyroid stimulation and involution.
  • Quantification of nuclear surface area, nuclear volume, total NPC number, and numerical density (Na).

    • Main Results:

    • Thyroid stimulation led to increased nuclear surface, volume, and total NPCs.
    • Numerical density (Na) of NPCs increased by day 6 of stimulation.
    • NPC parameters returned to normal during thyroid involution, suggesting correlation with cellular activity, not the cell cycle.
    • NPCs were randomly distributed, with neighboring complexes separated by 105 nm, and no clusters were observed.

    Conclusions:

    • NPC number and numerical density are linked to cellular activity rather than the cell cycle.
    • Two distinct mechanisms for NPC generation exist: membrane addition and de novo formation.
    • Nuclear membranes and NPCs are synchronously degraded during involution.
    • NPCs exhibit random distribution within the nuclear envelope.