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Automated estimation of factor Xa using the chromogenic substrate S-2222
Haemostasis
|January 1, 1981
Summary
A new automated method accurately measures factor Xa levels using a fast analyzer and a specific substrate. This technique overcomes fibrin interference, enabling reliable factor Xa estimation in plasma for clinical applications.
Area of Science:
- Clinical Chemistry
- Hematology
- Biochemical Assays
Background:
- Accurate measurement of factor Xa is crucial for monitoring coagulation.
- Existing methods for factor Xa determination can be time-consuming or subject to interference.
Purpose of the Study:
- To develop and validate a rapid, automated method for quantifying factor Xa.
- To address and overcome the issue of fibrin interference in factor Xa assays.
Main Methods:
- Utilized the "Centrifichem" rotary fast analyzer with the S-2222 substrate for factor Xa estimation.
- Implemented a centrifugation-based activation step to achieve simultaneous defibrination and factor X activation.
- Correlated the automated assay results with a standard clotting assay.
Main Results:
- Successfully developed a rapid automated assay for factor Xa estimation.
- The method effectively eliminated interference from fibrin by integrating activation and defibrination.
- Demonstrated good correlation between the automated assay and a clotting assay across normal and warfarin-treated patient plasmas.
Conclusions:
- The developed automated method provides a fast and reliable means for factor Xa determination.
- This assay is suitable for routine clinical use, including monitoring anticoagulant therapy.
- The integrated defibrination and activation step enhances assay robustness and efficiency.