Related Experiment Videos
Specific anti-thyroxine antisera induced by thyroxine sensitized liposomes
Specific anti-thyroxine rabbit antisera were generated from complete Freund's adjuvant with liposomes consisting of sphingomyelin, cholesterol, dicetylphosphate and 5-N-thyroxine-2,4-dinitrophenyl-phosphatidylethanolamine (T4-Dnp-PE). Spin membrane immunoassay technique was used to measure the sensitivity and specificity of these antisera. Addition of 10 to 70 ng of L-thyroxine produced significant inhibition of immune lysis. Addition of L-3,3',5-triiodothyronine (T3) up to 800 ng showed no cross reaction.
Specific anti-thyroxine rabbit antisera were generated from complete Freund's adjuvant with liposomes consisting of sphingomyelin, cholesterol, dicetylphosphate and 5-N-thyroxine-2,4-dinitrophenyl-phosphatidylethanolamine (T4-Dnp-PE). Spin membrane immunoassay technique was used to measure the sensitivity and specificity of these antisera. Addition of 10 to 70 ng of L-thyroxine produced significant inhibition of immune lysis. Addition of L-3,3',5-triiodothyronine (T3) up to 800 ng showed no cross reaction.