High pressure liquid chromatographic determination of nifursol in finished feeds

Nifursol is extracted from feed with acetonitrile, and then washed with carbon disulfide to remove feed interferences. An aliquot is concentrated, diluted with 5% NaCl solution, filtered, and then subjected to high pressure liquid chromatography on a strong anion exchange column, using 365 nm photometric detection. The average recovery of nifursol from fortified feed samples was 100.6% with an average coefficient of variation of 3.5%. The nifursol determination is not subject to interference from the other commonly used nitrofurans.