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Liquid chromatographic method for determination of arprinocid in feed: collaborative study
Journal - Association of Official Analytical Chemists
|July 1, 1981
Summary
An HPLC method for arprinocid determination in feed was validated. The reliable method demonstrated excellent accuracy and precision, leading to its official adoption.
Area of Science:
- Analytical Chemistry
- Food Science
- Veterinary Pharmacology
Background:
- Accurate quantification of feed additives is crucial for animal health and regulatory compliance.
- Arprinocid is an anticoccidial drug used in animal feed.
- A robust analytical method is required for its reliable determination in commercial feeds.
Purpose of the Study:
- To evaluate an High-Performance Liquid Chromatography (HPLC) method for determining arprinocid in animal feed.
- To assess the method's accuracy, precision, and reliability through an interlaboratory collaborative study.
Main Methods:
- Samples containing arprinocid (0.0050-0.0070%) were prepared and distributed to 14 laboratories.
- Arprinocid was extracted using chloroform and purified via liquid-liquid partitioning.
- Determination was performed using HPLC with a silica column and UV detection.
Main Results:
- Collaborators' results ranged from 97% to 104% of the true arprinocid concentration, showing no significant difference (P > 0.1).
- The average coefficient of variation was 6.8%, indicating good precision.
- Systematic error was not statistically significant (P > 0.1), confirming method accuracy.
Conclusions:
- The evaluated HPLC method is accurate and precise for determining arprinocid in feed.
- The method's reliability has been confirmed by the interlaboratory collaborative study.
- This validated HPLC method has been officially adopted for routine analysis.