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Determination of relative antigen-antibody affinities by quantitative immunoelectrophoresis
The relative affinities of an antibody population of different antigens or of different antibody populations for one antigen can be determined by quantitative immunoelectrophoresis. Antigen-antibody interactions of greater average affinity result in a greater increase in rocket area as a function of the amount of antigen applied to the wells. This is measured as the slope of the line in plots of rocket area versus antigen amount. Quantitative immunoelectrophoresis of different antigens, which nevertheless have the same affinity for antibody, produces plots with the same slopes. The relative magnitudes of the slopes of these lines reflect the relative average affinities of different antibody populations for an antigen.
The relative affinities of an antibody population of different antigens or of different antibody populations for one antigen can be determined by quantitative immunoelectrophoresis. Antigen-antibody interactions of greater average affinity result in a greater increase in rocket area as a function of the amount of antigen applied to the wells. This is measured as the slope of the line in plots of rocket area versus antigen amount. Quantitative immunoelectrophoresis of different antigens, which nevertheless have the same affinity for antibody, produces plots with the same slopes. The relative magnitudes of the slopes of these lines reflect the relative average affinities of different antibody populations for an antigen.