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Some effects of glucagon on chick embryo development
Journal of Embryology and Experimental Morphology
|April 1, 1981
Summary
Glucagon administration in chick embryos impacts survival, growth, and tissue development. Optimal dosages improved survival and altered blood sugar and glycogen levels, influencing pancreatic tissue growth.
Area of Science:
- Developmental Biology
- Endocrinology
- Animal Science
Background:
- Glucagon is a key hormone regulating glucose metabolism.
- Understanding glucagon's role in embryonic development is crucial for metabolic research.
Purpose of the Study:
- To investigate the effects of varying glucagon concentrations on chick embryo development.
- To analyze glucagon's impact on survival, weight, blood glucose, glycogen storage, and pancreatic histogenesis.
Main Methods:
- Chick embryos were injected with glucagon (1.16–300.0 µg/0.1 ml) on incubation days 8, 10, and 12.
- Evaluated survival rates, embryo weights, blood glucose, liver and tibiotarsus glycogen, and pancreatic alpha/beta cell development.
Main Results:
- Dosages of 37.5 and 150.0 µg/0.1 ml glucagon yielded the highest survival rates.
- Glucagon increased embryo weight, liver glycogen, and pancreatic beta tissue, while decreasing alpha tissue.
- Hyperglycemia was observed initially, followed by hypoglycemia or prolonged hyperglycemia depending on dosage and administration day.
Conclusions:
- Glucagon dosage, insulin secretion, and initial glycogen levels modulate its developmental effects.
- Glucagon influences embryonic growth, metabolic regulation, and pancreatic endocrine development.