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Related Experiment Videos

Nylon adherent antigen-specific rosette-forming T cells

R E Cone, R K Gershon, P W Askenase

    The Journal of Experimental Medicine
    |November 1, 1977
    PubMed
    Summary
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    Antigen-specific thymic-derived lymphocytes-forming rosettes (T-RFC) appear in mouse spleens after immunization. Their exact immune function is unknown, but they may amplify suppressor cell activity.

    Area of Science:

    • Immunology
    • Cellular Immunology
    • Immunoregulation

    Background:

    • Antigen immunization in mice induces spleen cells forming rosettes with erythrocytes (RFC).
    • Two types of RFCs exist: thymic-derived (T-RFC) and non-thymic-derived (B-RFC).
    • T-RFC are Thy 1+ and B-RFC are Thy 1-.

    Purpose of the Study:

    • To characterize antigen-specific T-RFC and investigate their role in the immune response.
    • To compare T-RFC induction with B-RFC induction.
    • To explore the potential function of T-RFC in immune regulation.

    Main Methods:

    • Intravenous immunization of mice with heterologous erythrocytes (RBC).
    • Isolation and characterization of T-RFC and B-RFC based on Thy 1 expression and nylon adherence.

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  • Assessment of T-RFC and B-RFC induction under varying antigen doses and with cyclophosphamide pretreatment.

    • Main Results:

    • Antigen-specific T-RFC appear in the spleen shortly after immunization.
    • T-RFC are less stable than B-RFC but both adhere to nylon.
    • Low antigen doses induce T-RFC, while high doses inhibit them and optimally induce B-RFC.
    • Cyclophosphamide pretreatment prevents high-dose inhibition of T-RFC.
    • T-RFC are unlikely to be essential for delayed-type hypersensitivity (DTH) reactions.

    Conclusions:

    • Immunization leads to the production of numerous antigen-specific T-RFC.
    • The precise functional role of T-RFC in the immune response remains undetermined.
    • T-RFC characteristics suggest a potential role in amplifying suppressor cell activity.