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Related Experiment Videos

Current experience with radioimmunoassay techniques for prostatic acid phosphatase

J F Cooper, W D Finkle

    The Prostate
    |January 1, 1980
    PubMed
    Summary

    Radioimmunoassay (RIA) for prostatic acid phosphatase (PAP) is more sensitive than enzymatic methods for detecting prostate cancer. While not specific enough alone for screening, RIA-PAP combined with urologic follow-up shows promise.

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    Area of Science:

    • Urology
    • Oncology
    • Clinical Chemistry

    Background:

    • Prostatic acid phosphatase (PAP) is a biomarker for prostate cancer.
    • Enzymatic methods for PAP measurement have limitations in sensitivity.
    • Radioimmunoassay (RIA) offers potentially higher sensitivity for PAP detection.

    Purpose of the Study:

    • To evaluate the sensitivity and specificity of RIA for serum PAP in detecting prostate cancer.
    • To assess the feasibility of using RIA-PAP as a screening tool for prostate cancer.
    • To compare RIA techniques with traditional enzymatic methods for PAP measurement.

    Main Methods:

    • Serum samples from 6,320 men over 45 were tested using a solid-phase RIA for PAP.
    • Elevated PAP levels triggered clinical recall and urologic review.

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  • Suspected cases underwent prostatic needle biopsy for confirmation.

    • Main Results:

    • RIA demonstrated higher sensitivity for detecting all stages of prostate cancer compared to enzymatic methods.
    • 7% of screened men had elevated PAP levels via RIA.
    • Of those with elevated results, 88% were confirmed positive for prostate cancer after biopsy, indicating high specificity when combined with urologic follow-up.

    Conclusions:

    • RIA for PAP is a sensitive method for prostate cancer detection.
    • Isolated RIA-PAP testing is not specific enough for population screening due to false positives.
    • Combining RIA-PAP with urologic examination is a specific and promising approach for prostate cancer screening.