Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gel electrophoretic separation of globin chains

B P Alter

    Progress in Clinical and Biological Research
    |January 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a rapid gel electrophoresis method to separate human hemoglobin (Hb) chains, crucial for understanding globin composition and synthesis in various developmental stages and diseases like thalassemia.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    VACTERL-H Association and Fanconi Anemia.

    Molecular syndromology·2013
    Same author

    Disease-specific hematopoietic cell transplantation: nonmyeloablative conditioning regimen for dyskeratosis congenita.

    Bone marrow transplantation·2010
    Same author

    Oral and dental phenotype of dyskeratosis congenita.

    Oral diseases·2008
    Same author

    Griscelli syndrome: rare neonatal syndrome of recurrent hemophagocytosis.

    Journal of pediatric hematology/oncology·2002
    Same author

    Modern review of congenital hypoplastic anemia.

    Journal of pediatric hematology/oncology·2001
    Same author

    Comparative genome analysis delimits a chromosomal domain and identifies key regulatory elements in the alpha globin cluster.

    Human molecular genetics·2001

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Hematology

    Background:

    • Human hemoglobins (Hb) consist of various globin chains (embryonic, fetal, adult) that change during development.
    • Understanding the composition and synthesis of these globin chains is vital for diagnosing and studying hematological disorders.

    Purpose of the Study:

    • To develop and validate a simple, rapid, and cost-effective electrophoresis technique for separating and analyzing human globin chains.
    • To investigate globin chain composition and synthesis ratios in different developmental stages and disease states.

    Main Methods:

    • Electrophoresis on polyacrylamide gels containing acid, urea, and Triton X-100.
    • Analysis of protein composition using Coomassie blue-stained gels.
    • Measurement of protein synthesis via fluorograms of gels with 3H-leucine-labelled material.

    Related Experiment Videos

  • Utilized K562 erythroleukemia cells and erythroid colonies for synthesis studies.

    • Main Results:

    • Established the electrophoretic order of major human erythrocyte proteins: zeta, epsilon, carbonic anhydrase, A gamma, delta and G gamma (together), beta, and alpha.
    • Examined G gamma/A gamma ratios in fetuses and newborns, suggesting developmental switches may be independent.
    • Determined beta/gamma synthetic ratios in fetuses at risk for thalassemia.
    • Measured embryonic and fetal globin synthesis in K562 cells and erythroid colonies.

    Conclusions:

    • The developed gel electrophoresis method is a rapid, simple, and inexpensive approach for studying globin composition and synthesis.
    • The technique aids in analyzing globin chain ratios, essential for understanding hemoglobinopathies and developmental hematology.
    • Provides insights into the regulation of globin gene expression during human development and in disease contexts.