Simplified typing of mouse hemoglobin (Hbb) phenotypes using cystamine
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Updated: Aug 3, 2026
09:37
A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging
Published on: July 14, 2016
Biochemical Genetics
|August 1, 1978
Abstract:
Cellulose acetate electrophoresis of mouse hemoglobins modified with the disulfide reagent cystamine permits rapid, unequivocal discrimination of all combinations of the codominant mouse hemoglobin "single" (Hbbs) and "diffuse" (Hbbd and Hbbp) alleles. The single, diffuse major, diffuse d-minor, and diffuse p-minor adult hemoglobins are all resolved by this method, which depends on the presence of a cysteine in the beta chains of "diffuse" mice which is not found in the beta chain of "single" mice.
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