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A technique for detection and relative quantitative analysis of glucosephosphate isomerase isozymes from nanogram
Biochemical Genetics
|August 1, 1978
Abstract:
An improved method for detecting and measuring the enzyme glucosephosphate isomerase after starch gel electrophoresis is described. Nitrocellulose filters are used in a gel overlay procedure which increases the sensitivity of the staining reaction and provides a simple means for accurate quantitation of the isozyme pattern. This staining technique may have wider application with other gel media and also with other enzymes.