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Related Concept Videos

Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

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Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
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Mass Spectrometers01:16

Mass Spectrometers

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This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
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MALDI-TOF Mass Spectrometry01:19

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Mass Spectrometry: Complex Analysis01:21

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
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Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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Analyzing Large Protein Complexes by Structural Mass Spectrometry
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Tandem mass spectrometry

F W McLafferty

    Science (New York, N.Y.)
    |October 16, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Tandem mass spectrometry offers a sensitive and specific method for analyzing organic compounds in complex mixtures. This technique achieves rapid, picogram-level detection with high accuracy, outperforming other analytical methods.

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    Area of Science:

    • Analytical Chemistry
    • Organic Chemistry
    • Spectrometry

    Background:

    • Analysis of specific organic compounds in complex mixtures presents significant challenges.
    • Existing methods may lack the required sensitivity, specificity, or speed.

    Purpose of the Study:

    • To introduce and evaluate a novel technique for analyzing specific organic compounds.
    • To demonstrate the advantages of coupling mass spectrometers in series.

    Main Methods:

    • Utilizing tandem mass spectrometry (MS/MS) by coupling mass spectrometers in series.
    • Selective ionization of target compounds followed by separation of characteristic ions in the first mass spectrometer.
    • Collision-induced decomposition of selected ions and analysis of product ions in the second mass spectrometer.

    Main Results:

    • Achieved picogram-level sensitivity for targeted compounds.
    • Demonstrated high specificity and nearly instantaneous response.
    • Tandem mass spectrometry showed comparable specificities and sensitivities to radioimmunoassay and GC/MS.

    Conclusions:

    • Coupled mass spectrometry is a powerful technique for analyzing specific organic compounds in complex matrices.
    • The method offers significant advantages in terms of speed, sensitivity, and specificity.
    • Tandem mass spectrometry provides a viable alternative to established analytical techniques.