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Related Experiment Videos

Bioselective membrane electrode probes

G A Rechnitz

    Science (New York, N.Y.)
    |October 16, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Intact cells and tissue slices offer a broader range of applications for potentiometric bioselective electrodes compared to traditional enzyme electrodes. These biocatalysts provide cost-effective, stable, and easy-to-prepare alternatives for various biosensing needs.

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    Area of Science:

    • Biotechnology
    • Biosensor Development
    • Electrochemistry

    Background:

    • Conventional enzyme electrodes are limited in their range of applications.
    • Isolated enzymes are not always available or suitable for complex reactions.

    Purpose of the Study:

    • To explore the use of intact bacterial cells and plant/animal tissue slices as immobilized biocatalysts.
    • To expand the capabilities of potentiometric bioselective membrane electrodes.

    Main Methods:

    • Immobilization of intact bacterial cells.
    • Immobilization of plant and animal tissue slices.
    • Development of potentiometric bioselective membrane electrodes.

    Main Results:

    • Expanded the range of potentiometric bioselective membrane electrodes beyond conventional enzyme electrodes.

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  • Demonstrated the utility of intact cells and tissue slices as effective biocatalysts.
  • Achieved exceptional ease of preparation, time stability, and low cost.

    • Conclusions:

    • Intact bacterial cells and tissue slices are viable and advantageous biocatalysts for potentiometric bioselective electrodes.
    • These novel biocatalysts offer a cost-effective and stable alternative to isolated enzymes.
    • The use of intact biological materials broadens the scope of biosensor applications.