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[Washed erythrocyte concentrate, degree of purity and cell vitality]
The reduction of leukocytes of packed red cells amounted to 70 +/- 8% (n = 29) by single removing of buffy coat and to 86 +/- 9% (n = 12) by additional washing in isotonic NaCl-solution with a loss of 23% of red cells. The ratio of granulocytes to lymphocytes increased The ratio of granulocytes to lymphocytes increased slightly by washing. Storage of washed red cells damaged their function in dependence on temperature and storage medium more than the washing procedure. The ATP content of washed red cells maintained at the normal level during 24 hr at 277 K (4 degrees C), but the 2.3-P2G level decreased to 1/3 of the normal value. The survival rate of washed red cells amounted to 86 +/- 7% (n = 7). The preparation of red cells poor in leukocytes in recommended by removing buffy coat and the upper 50 ml of red cells after single centrifugation of blood and adding 100 ml CDS-AG (citrate-glucose-sucrose-adenine-guanosine) preservation solution of packed red cells. A storage of 6 weeks is possible. Thus washing the red cells in isotonic NaCl-solution for the purpose of leukocyte reduction can be omitted.
The reduction of leukocytes of packed red cells amounted to 70 +/- 8% (n = 29) by single removing of buffy coat and to 86 +/- 9% (n = 12) by additional washing in isotonic NaCl-solution with a loss of 23% of red cells. The ratio of granulocytes to lymphocytes increased The ratio of granulocytes to lymphocytes increased slightly by washing. Storage of washed red cells damaged their function in dependence on temperature and storage medium more than the washing procedure. The ATP content of washed red cells maintained at the normal level during 24 hr at 277 K (4 degrees C), but the 2.3-P2G level decreased to 1/3 of the normal value. The survival rate of washed red cells amounted to 86 +/- 7% (n = 7). The preparation of red cells poor in leukocytes in recommended by removing buffy coat and the upper 50 ml of red cells after single centrifugation of blood and adding 100 ml CDS-AG (citrate-glucose-sucrose-adenine-guanosine) preservation solution of packed red cells. A storage of 6 weeks is possible. Thus washing the red cells in isotonic NaCl-solution for the purpose of leukocyte reduction can be omitted.