Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

What are Membranes?01:54

What are Membranes?

190.3K
A key characteristic of life is the ability to separate the external environment from the internal space. To do this, cells have evolved semi-permeable membranes that regulate the passage of biological molecules. Additionally, the cell membrane defines a cell’s shape and interactions with the external environment. Eukaryotic cell membranes also serve to compartmentalize the internal space into organelles, including the endomembrane structures of the nucleus, endoplasmic reticulum and...
190.3K
SNAREs and Membrane Fusion01:43

SNAREs and Membrane Fusion

12.7K
Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion.
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
12.7K
Fusion of Secretory Vesicles with the Plasma Membrane01:26

Fusion of Secretory Vesicles with the Plasma Membrane

18.9K
Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
18.9K
Assessing Body Temperature - Tympanic membrane01:14

Assessing Body Temperature - Tympanic membrane

1.2K
Assessing tympanic membrane temperature involves using a tympanic membrane thermometer (TMT). Here is a step-by-step guide:
Step 1: Begin by practicing good hand hygiene to prevent the transmission of microorganisms.
Step 2: Turn on the thermometer and wait until the ready sign appears on the screen to ensure accurate measurement.
Step 3: Slide the probe cover in place to prevent cross-contamination.
Step 4: Instruct the patient to tilt their head to the side for comfort and check for cerumen...
1.2K
Introduction to Membrane Proteins01:16

Introduction to Membrane Proteins

81.0K
The cell membrane, or plasma membrane, is an ever-changing landscape. It is described as a fluid mosaic where various macromolecules are embedded in the phospholipid bilayer. Among the macromolecules are proteins. The protein content varies across cell types. For example, mitochondrial inner membranes contain ~76% protein content, while myelin contains ~18% protein content. Individual cells contain many types of membrane proteins—red blood cells contain over 50—and different cell...
81.0K
What are Membranes?01:24

What are Membranes?

18.8K
A cell's plasma membrane demarcates the cell's borders and determines the nature of its interaction with the environment. Cells exclude certain substances, take in others, and excrete some others in controlled quantities. The plasma membrane must be flexible to allow certain cells, such as red and white blood cells, to change their shape while passing through narrow capillaries. These are the more obvious plasma membrane functions. In addition, the plasma membrane's surface carries...
18.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Glycaemia and autistic traits in very low birth weight infants in adulthood.

Diabetes & metabolism·2016
Same author

Normal contact and friction of rubber with model randomly rough surfaces.

Soft matter·2014
Same author

Noise-activated dissociation of soft elastic contacts.

The European physical journal. E, Soft matter·2012
Same author

Vibration spectroscopy of a sessile drop and its contact line.

Langmuir : the ACS journal of surfaces and colloids·2012
Same author

Random motion with interfacial contact: driven diffusion vis-à-vis mechanical activation.

The European physical journal. E, Soft matter·2012
Same author

Stochastic rolling of a rigid sphere in weak adhesive contact with a soft substrate.

The European physical journal. E, Soft matter·2011

Related Experiment Video

Updated: Jan 30, 2026

A Model Membrane Platform for Reconstituting Mitochondrial Membrane Dynamics
10:31

A Model Membrane Platform for Reconstituting Mitochondrial Membrane Dynamics

Published on: September 2, 2020

8.1K

Correlation between membrane expansion and temperature-induced membrane fusion

M K Chaudhury, S Ohki

    Biochimica Et Biophysica Acta
    |April 6, 1981
    PubMed
    Summary

    Phospholipid membranes expand similarly between phase transition and fusion temperatures. Cholesterol incorporation inhibits this expansion and membrane fusion, while pH affects fusion by altering phase transition temperatures.

    More Related Videos

    A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion
    07:22

    A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion

    Published on: August 14, 2018

    7.0K
    Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay
    09:19

    Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay

    Published on: October 19, 2012

    14.4K

    Related Experiment Videos

    Last Updated: Jan 30, 2026

    A Model Membrane Platform for Reconstituting Mitochondrial Membrane Dynamics
    10:31

    A Model Membrane Platform for Reconstituting Mitochondrial Membrane Dynamics

    Published on: September 2, 2020

    8.1K
    A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion
    07:22

    A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion

    Published on: August 14, 2018

    7.0K
    Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay
    09:19

    Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay

    Published on: October 19, 2012

    14.4K

    Area of Science:

    • Membrane biophysics
    • Lipid bilayer thermodynamics

    Background:

    • Phospholipid membranes exhibit distinct phase transition and fusion temperatures.
    • Understanding the relationship between membrane expansion and fusion is crucial for membrane dynamics.

    Purpose of the Study:

    • To investigate the correlation between temperature-induced membrane fusion and membrane expansion.
    • To explore the influence of cholesterol and pH on phospholipid membrane physical states.

    Main Methods:

    • Utilized a monolayer system to study phospholipid membrane expansion.
    • Investigated the effect of temperature changes from phase transition to fusion temperature.
    • Examined the impact of cholesterol and pH on membrane properties.

    Main Results:

    • Monolayer expansion was consistent across five phospholipids when temperature increased from phase transition to fusion.
    • Cholesterol incorporation significantly reduced membrane expansion and correlated with inhibited fusion.
    • pH changes affected fusion temperature by shifting phase transition temperatures, with expansion remaining consistent across surface charges.

    Conclusions:

    • Membrane expansion between phase transition and fusion temperatures is a conserved property of phospholipids.
    • Cholesterol acts as a fusion inhibitor by restricting membrane expansion.
    • pH-induced shifts in phase transition temperatures explain observed changes in fusion temperatures.