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Antithrombin III does not have bound glucocerebroside

G L Dale, B Westwood

    Biochimica Et Biophysica Acta
    |July 28, 1981
    PubMed
    Summary

    Previous research suggested antithrombin III binds plasma glucocerebroside. However, this study found glucocerebroside and antithrombin III are in separate plasma fractions, indicating no association.

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    Area of Science:

    • Biochemistry
    • Lipid Metabolism
    • Protein-Lipid Interactions

    Background:

    • Plasma contains glucocerebroside, a major glycolipid.
    • Antithrombin III is a key protein involved in blood coagulation.
    • Previous reports suggested a binding interaction between antithrombin III and glucocerebroside.

    Purpose of the Study:

    • To investigate the association between purified antithrombin III and glucocerebroside in plasma.
    • To determine if glucocerebroside is bound to antithrombin III in native plasma.

    Main Methods:

    • Plasma separation using ultracentrifugation into lipoprotein-rich and lipid-deficient fractions.
    • Analysis of fraction composition to identify glucocerebroside and antithrombin III distribution.

    Main Results:

    • Glucocerebroside was found in lipoprotein-rich plasma fractions.
    • Antithrombin III was primarily located in lipid-deficient plasma fractions.
    • No evidence of glucocerebroside association with antithrombin III was observed.

    Conclusions:

    • Glucocerebroside and antithrombin III exist in distinct fractions within plasma.
    • The previously reported binding of glucocerebroside to antithrombin III is not supported by these findings.
    • Antithrombin III does not appear to have glucocerebroside associated with it in plasma.

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