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Related Experiment Videos

A vegetalizing inducing factor. Isolation and chemical properties

H P Geithe, M Asashima, K I Asahi

    Biochimica Et Biophysica Acta
    |September 4, 1981
    PubMed
    Summary

    Researchers purified a vegetalizing factor from chicken embryos that induces endodermal and mesodermal organ formation in amphibian ectoderm. This factor

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    Area of Science:

    • Developmental Biology
    • Molecular Biology

    Background:

    • Ectoderm of amphibian gastrula can form endodermal and mesodermal organs.
    • A vegetalizing factor from chicken embryos induces this transformation.

    Purpose of the Study:

    • To purify the vegetalizing factor from chicken embryos.
    • To characterize the factor's molecular properties and optimize purification methods.

    Main Methods:

    • Preparative sodium dodecyl sulfate polyacrylamide electrophoresis.
    • Gel permeation chromatography on Sephadex with various eluants.
    • High-performance gel permeation chromatography using formic acid.

    Main Results:

    • The factor's molecular weight was estimated to be 28,000-30,000 in 6 M urea.

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  • Partial dissociation into smaller chains occurred in sodium dodecyl sulfate (SDS) buffers.
  • Complete dissociation into 13,000-15,000 molecular weight peptides was observed in 50%-70% formic acid.
  • Formic acid minimized factor absorption to matrices, enabling effective purification.

    • Conclusions:

    • A vegetalizing factor from chicken embryos was purified.
    • Formic acid is a suitable eluant for the factor's purification due to minimal absorption and complete dissociation.
    • The factor's properties were characterized, aiding further research into developmental processes.