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Updated: May 11, 2026

Quantification of the Abundance and Charging Levels of Transfer RNAs in Escherichia coli
Published on: August 22, 2017
Mutants of Su+7 tRNA include a functional tRNA with an altered T pseudo uracil CG sequence
Abstract:
Su+7 tRNA, the amber-suppressing anticodon mutant of tRNA(Trp) (E. coli), also relaxes stringent control. Further mutant Su+7 tRNAs have been isolated that have lost this regulatory effect. The sequence changes we have observed are a unique set. They include partially active suppressor tRNAs with a C to A change in the T pseudo uracil CG common sequence and in the anticodon stem. The activity of the T pseudo uracil CG and anticodon-stem mutants suggests that neither sequence is essential for ribosomal function. The third mutated site, a D-stem alteration, dramatically disturbs tRNA maturation. The mutations add to our knowledge of tRNA function, particularly with regard to the dispensability of the T pseudo uracil CG sequence for activity at the ribosomal A site.
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