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Related Experiment Videos

Direct memory access of diffraction patterns from striated muscle--a software view

R L Lieber, R J Baskin

    Computer Programs in Biomedicine
    |March 1, 1981
    PubMed
    Summary
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    New software enables direct memory access (DMA) microprocessor systems to store and analyze muscle diffraction data. This facilitates research into muscle striation spacing and force generation mechanisms.

    Area of Science:

    • Biophysics
    • Muscle Physiology
    • Instrumentation

    Background:

    • Striated muscle fibers exhibit regular light and dark regions.
    • These regions act as phase gratings for laser light, producing diffraction patterns.
    • Diffraction data analysis can reveal insights into muscle striation spacing and force generation.

    Purpose of the Study:

    • To develop comprehensive software for a direct memory access (DMA) microprocessor system.
    • To enable efficient storage and analysis of diffraction data from striated muscle.
    • To facilitate sophisticated diffraction experiments for studying muscle mechanics.

    Main Methods:

    • Utilized a charge-coupled device (CCD) for detecting diffracted order, position, and intensity.
    • Integrated a high-speed muscle puller and tension transducer for mechanical characterization.

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  • Developed interleaved DMA and control loop cycles for efficient data acquisition and processing.

    • Main Results:

    • Achieved digitization and storage of full 256-point CCD analog output in approximately 2 ms.
    • Enabled direct data transfer from CCD to memory, freeing the CPU for experimental control.
    • Created user-friendly software for inexperienced users to conduct complex diffraction experiments.

    Conclusions:

    • The developed software system significantly enhances the capability to study muscle diffraction.
    • The system provides rapid data acquisition and processing for muscle mechanics research.
    • It allows for detailed analysis of muscle striation spacing and its relation to force generation.