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Separative determination of ascorbic acid and erythorbic acid by high-performance liquid chromatography
Journal of Nutritional Science and Vitaminology
|January 1, 1981
Summary
This study presents a rapid High-Performance Liquid Chromatography (HPLC) method for accurately quantifying ascorbic acid (AsA) and erythorbic acid (ErA). The developed technique offers distinct separation and high sensitivity for these vital compounds.
Area of Science:
- Analytical Chemistry
- Biochemistry
Background:
- Ascorbic acid (AsA) and erythorbic acid (ErA) are important compounds with various applications.
- Accurate and efficient quantification methods are crucial for their analysis.
Purpose of the Study:
- To develop and validate a High-Performance Liquid Chromatography (HPLC) method for the simultaneous determination of AsA and ErA.
- To establish a sensitive and rapid analytical technique for these compounds.
Main Methods:
- High-Performance Liquid Chromatography (HPLC) using a Shimadzu LC-2P system.
- UV detection at 254 nm with a LiChrosorb-NH2 column.
- Elution with a mobile phase comprising acetonitrile, acetic acid, and water (87:2:11, v/v).
Main Results:
- Achieved distinct separation of AsA and ErA.
- Demonstrated a short analysis time per sample.
- Established a detection limit as low as 1.0 x 10(-2) microgram.
- Obtained satisfactory recovery rates for dehydro-AsA and dehydro-ErA after H2S reduction.
Conclusions:
- The developed HPLC method is effective for the precise quantification of AsA and ErA.
- The method is advantageous due to its speed, sensitivity, and distinct separation capabilities.
- This technique provides a reliable tool for analyzing AsA and ErA in various samples.