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Updated: May 8, 2026

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
09:02

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

Trans-complementation-dependent replication of a low molecular weight origin fragment from plasmid R6K

R Kolter, M Inuzuka, D R Helinski

    Cell
    |December 1, 1978
    PubMed
    Summary

    Researchers identified a functional origin of replication in plasmid R6K, crucial for initiating plasmid DNA replication. This discovery aids in understanding and manipulating plasmid replication mechanisms in bacteria.

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