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Nutritional physiology of pathogenic species of thermophilic mucor

V W Ogundero

    Sabouraudia
    |September 1, 1981
    PubMed

    Insights

    Optimal growth conditions for zoopathogenic fungi Mucor pusillus and Mucor miehei were identified. Key factors include a pH of 5.0-6.0, specific carbon and nitrogen sources, and defined concentrations for growth and sporulation.

    Area of Science:

    • Mycology
    • Microbial Physiology
    • Zoopathology

    Background:

    • Zoopathogenic fungi Mucor pusillus and Mucor miehei are significant in animal health.
    • Understanding their nutritional physiology is crucial for controlling infections and optimizing industrial applications.
    • Previous research has not fully elucidated the specific nutritional requirements for optimal growth and sporulation of these species.

    Purpose of the Study:

    • To investigate the nutritional physiology of Mucor pusillus and Mucor miehei at 37°C.
    • To determine optimal conditions for growth and asexual sporulation (sporangiospores).
    • To identify ideal carbon and nitrogen sources, pH, and nutrient concentrations for these fungi.

    Main Methods:

    • Culturing of Mucor pusillus and Mucor miehei on various media at 37°C.
    • Testing different carbon sources (including dextrin, starch, and carboxymethyl cellulose), nitrogen sources (including casein hydrolysate and DL-glutamine), and L-sorbose and DL-tryptophan.
    • Evaluating the impact of pH (5.0-6.0), glucose and DL-glutamine concentrations (15.0-20.0 gl⁻¹ and 5.0-7.5 gl⁻¹, respectively), and C/N ratios on fungal growth and sporulation.

    Main Results:

    • Both species produced only asexual sporangiospores under tested conditions.
    • Optimal pH range for growth and sporulation was 5.0-6.0.
    • Dextrin and starch were superior carbon sources; casein hydrolysate was the preferred nitrogen source. Soluble carboxymethyl cellulose supported growth and sporulation. Optimal glucose and DL-glutamine concentrations were 15.0-20.0 gl⁻¹ and 5.0-7.5 gl⁻¹, respectively. Optimal C/N ratios were 15.0:5.0 gl⁻¹ for sporulation and 20.07:7.5 gl⁻¹ for mycelial growth.

    Conclusions:

    • Mucor pusillus and Mucor miehei exhibit specific nutritional requirements for optimal growth and sporulation.
    • The study provides precise parameters for cultivating these fungi, essential for both research and potential biotechnological applications.
    • Understanding these physiological aspects aids in managing zoopathogenic infections and exploring the fungi's industrial potential.

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