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Transmissible plasmids from Campylobacter jejuni

D E Taylor, S A De Grandis, M A Karmali

    Antimicrobial Agents and Chemotherapy
    |May 1, 1981
    PubMed
    Summary
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    Tetracycline resistance in Campylobacter jejuni is plasmid-mediated and transferable to other species. Transfer efficiency is significantly higher via solid-surface methods compared to liquid methods.

    Area of Science:

    • Microbiology
    • Genetics
    • Antimicrobial Resistance

    Background:

    • Tetracycline resistance is a growing concern in clinical pathogens.
    • Understanding resistance mechanisms in Campylobacter jejuni is crucial for public health.
    • Previous studies suggested plasmid involvement in Campylobacter resistance.

    Purpose of the Study:

    • To investigate the mechanism of tetracycline resistance in Campylobacter jejuni.
    • To determine the transmissibility of tetracycline resistance plasmids within and between Campylobacter species.
    • To elucidate the method of plasmid transfer in Campylobacter jejuni.

    Main Methods:

    • Plasmid DNA was isolated from tetracycline-resistant Campylobacter jejuni strains.
    • Conjugation experiments were performed using plate-mating, filter-mating, and liquid-mating methods.

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  • Interspecies and intraspecies transfer experiments were conducted with Campylobacter fetus subsp. fetus.
  • Transduction and transformation were assessed using cell-free filtrates and DNase treatment.

    • Main Results:

    • Tetracycline resistance in Campylobacter jejuni was confirmed to be plasmid-mediated.
    • Efficient intra- and interspecies transfer of resistance to Campylobacter fetus subsp. fetus was demonstrated.
    • Solid-surface mating methods (plate- and filter-mating) increased transfer frequency by approximately 100-fold compared to liquid mating.
    • Evidence suggests that bacteriophage-mediated transduction and transformation are not the primary mechanisms for tetracycline resistance transfer.
    • Four transmissible plasmids, each with a molecular weight of 38 x 10^6, were identified.
    • Transfer of these plasmids to Escherichia coli was not observed.

    Conclusions:

    • Plasmid-mediated transfer is the primary mechanism for tetracycline resistance dissemination in Campylobacter jejuni.
    • Optimized mating conditions significantly enhance plasmid transfer efficiency.
    • The identified plasmids are transmissible within Campylobacter but not readily to Gram-negative bacteria like E. coli.
    • Further research is needed to understand the broader implications of this resistance mechanism.