Microbial formation of secondary and tertiary amines in municipal sewage

Dimethylamine was formed in municipal sewage that was amended with creatinine, and trimethylamine was formed from choline or phosphatidylcholine. The maximum level of product that accumulated was equivalent to 0.13, 0.096, and 6.7% by weight, respectively, of the added chemicals. No dimethylamine or trimethylamine was detected in sewage amended with betaine, and no dimethylamine was found in sewage that was amended with methylamine and methionine.