Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Epoxide hydrolase activity in human skin

V A O'Neill, M D Rawlins, P H Chapman

    British Journal of Clinical Pharmacology
    |October 1, 1981
    PubMed
    Summary

    Epoxide hydrolase (EH) activity is present in human skin, including epidermis and dermis. While not significantly impacting systemic epoxide metabolism, skin EH may influence local epidermal effects.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Three Cases of Snake-Bite and Antivenene.

    The Indian medical gazette·2017
    Same author

    Interdose control of beta-blockade and arterial blood pressure during chronic oral labetalol treatment.

    British journal of clinical pharmacology·2015
    Same author

    A comparative study of methyldopa and labetalol in the treatment of hypertension.

    British journal of clinical pharmacology·2015
    Same author

    Mean steady-state plasma concentrations of labetalol in patients undergoing antihypertensive therapy.

    British journal of clinical pharmacology·2015
    Same author

    A multicentre open trial of labetalol in New Zealand.

    British journal of clinical pharmacology·2015
    Same author

    Spontaneous reporting of fatal adverse drug reactions.

    The International journal of risk & safety in medicine·2013

    Area of Science:

    • Biochemistry
    • Dermatology
    • Toxicology

    Background:

    • Epoxide hydrolase (EH) enzymes play a crucial role in metabolizing epoxides, which are reactive compounds.
    • Understanding EH activity in skin is important due to potential environmental or endogenous epoxide exposure.
    • Skin's role in systemic epoxide metabolism and its own localized effects requires investigation.

    Purpose of the Study:

    • To quantify epoxide hydrolase (EH) activity in human skin biopsies.
    • To determine if EH activity differs between skin layers (epidermis and dermis) or anatomical sites.
    • To assess the potential contribution of skin EH to systemic epoxide metabolism versus localized effects.

    Main Methods:

    • Human skin biopsies (n=42) were used to measure epoxide hydrolase (EH) activity.
    • 7-[H3]-styrene oxide served as the substrate for EH assays.
    • High-performance liquid chromatography (HPLC) was employed for product separation and quantification.

    Main Results:

    • Significant epoxide hydrolase (EH) activity was detected in both separated epidermis and dermis.
    • Mean EH activity was 139 +/- 105 pmol glycol/mg/min in epidermis and 165 +/- 120 pmol glycol/mg/min in dermis.
    • Whole skin EH activity varied widely (433 +/- 254 pmol glycol/mg/min) with no significant differences across breast, penis, or leg skin sites.

    Conclusions:

    • Epoxide hydrolase (EH) activity is demonstrably present in human skin.
    • Skin EH activity appears to have a limited role in the overall systemic metabolism of epoxides.
    • Skin EH may be more critical in modulating the biological impact of epoxides generated directly within the epidermis.

    Related Experiment Videos