An immunological analysis of the effects of bromelain on the mouse erythrocyte membrane
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The technique of crossed immunoelectrophoresis was used to analyse the effects of bromelain on the antigens contained within the membranes of mouse erythrocytes. Immune sera were obtained from goats that had been injected with membranes prepared from either normal erythrocytes or bromelain-modified erythrocytes. Precipitation patterns obtained by crossed immunoelectrophoresis showed that treatment of erythrocytes with bromelain changed the electrophoretic mobility of a major protein antigen and increased the solubility of two membrane glycoproteins. The possibility that enzymic modification of the erythrocytes led to the creation of entirely new antigenic determinants was not supported by the precipitin patterns obtained using sera absorbed with various membrane preparations.
The technique of crossed immunoelectrophoresis was used to analyse the effects of bromelain on the antigens contained within the membranes of mouse erythrocytes. Immune sera were obtained from goats that had been injected with membranes prepared from either normal erythrocytes or bromelain-modified erythrocytes. Precipitation patterns obtained by crossed immunoelectrophoresis showed that treatment of erythrocytes with bromelain changed the electrophoretic mobility of a major protein antigen and increased the solubility of two membrane glycoproteins. The possibility that enzymic modification of the erythrocytes led to the creation of entirely new antigenic determinants was not supported by the precipitin patterns obtained using sera absorbed with various membrane preparations.