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[Relation between changes in platelet shape, calcium balance and the separating effect of cytochalasin B]
Abstract:
Platelets were stimulated in vitro by different ADP concentration (10(-7)--10(-9) M). Scanning electron microscopy, light transmission recording and the Ca-sensitive fluorescent probe, chlortetracycline, were used to study the time course of changes in the shape, aggregation and the amount of membrane-bound calcium during ADP activation. It was found that ADP (10(-7)--10(-8)M) causes reversible changes in the shape of platelets without disturbing their aggregation. The changes in question were accompanied by phasic alterations of the amount of membrane-bound calcium.
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