Determination of trace elements in human serum by energy dispersive X-ray fluorescence

A system of energy dispersive X-ray fluorescence spectrometry (EDXRF) has been described to measure the concentrations of iron, copper, zinc, bromine, selenium and arsenic in human serum of healthy adults. Methods of sample preparations, standardization, and analysis have been presented. Data have been presented to show the accuracy, precision and sensitivity of the EDXRF system. The major advantages of using this system for the determination of trace elements in biological tissues are the relatively small sample size, ease of sample preparation, and the ability to perform rapid multi-element analysis on a single sample. The need for multi-element analysis has become apparent from the increased knowledge of trace element interactions in biological systems. The EDXRF system provides a solution for this need. Although the present system was applied only to the measurement of six elements, in principle the concentrations of V, Cr, Mn, Ni and Ti could also be routinely determined with improvements in technology and sensitivity.