Stimulation by tubulin of an adenylate cyclase from murine plasmacytoma

Different brain tubulin preparations were shown to stimulate membrane-bound adenylate cyclase from the murine plasmacytoma MOPC 173. Purified tubulin devoid of microtubule-associated proteins and of nucleoside-diphosphate kinase activity was responsible for this stimulation. Activation of the basal adenylate cyclase activity occurred in less than 2 min at 32 degrees C and was amplified by a 4 degree C preincubation of tubulin with plasma membranes. Tubulin affected the Km and the V of the enzyme and was shown to be associated with the membrane during the activation phenomenon. Tubulin was more active on the basal adenylate cyclase activity than that stimulated by fluoride or guanosine 5'-[beta, gamma-imido]triphosphate. GTP has no effect on the tubulin-stimulated enzyme.