Hypothalamic regulation of lipid metabolism in the rat: effect of hypothalamic stimulation on lipolysis

In unanesthetized rats, electrical stimulation of the ventromedial hypothalamic nucleus (VMH) induced a marked increase in plasma concentration of glycerol, but did not increase the plasma free fatty acid (FFA) concentration, probably owing to a great elevation of plasma lactate which might inhibit the release of FFA from adipose tissue. In anesthetized rats, on stimulation of the VMH there was no remarkable increase in the plasma lactate, and the plasma glycerol and FFA concentrations were both elevated markedly. Electrical stimulation of the lateral hypothalamic nucleus (LH), on the other hand, had no significant effects on plasma glycerol and FFA levels. Bilateral adrenodemedullation did not prevent the lipolytic response to VMH stimulation, although it reduced slightly the increment of plasma glycerol and FFA. However, the lipolytic response was completely blocked by previous treatment of the animals with hexamethonium or propranolol, but not with phentolamine. These results suggest that sympathetic innervation of the adipose tissue is the dominant factor involved in VMH-induced lipolysis in the rat, while the role of the adrenal medulla is subdominant; the effect of VMH stimulation is mainly transmitted through the sympathetic nervous system to beta-adrenergic receptor of the adipose tissue.