[Growth of some pathogenic microorganisms on chemically defined media which were proposed for sensitivity testing...

The growth of 16 bacterial cultures, which belong to the frequently encountered pathogens, in three different chemically defined fluid media was investigated through turbidity measurements in comparison with Mueller-Hinton-broth. E. coli, Salm. dublin, Salm. typhi-murium, Enterobacter cloacae, Proteus mirabilis, Pseud. aeruginosa and Bac. cereus grew in two of the three tested defined media with approximately the same intensity as in the Mueller-Hinton broth. Staph. aureus grew well only in one of the 3 tested defined media. Listeria monocytogenes, Erysipelothrix rhusiopathiae, Pasteurella multocida, Neisseria gonorrhoeae and Brucella abortus showed only in one of the three tested media a barely descernible growth, whereas Strept. faecalis, Strept. pneumoniae, Strept. pyogenes showed no growth in the three tested defined media after 34 hours of incubation. None of the tested defined media can be used for routine sensitivity testing but the use of two of the three tested defined media as a reference medium for the standardization of the complex sensitivity testing media remains theoretically possible.