Incorporation of chemically modified proteins into liposomes

Enzymes, not anchored into bilayers of membranes like trypsin, alpha-chymotrypsin, and carboxypeptidase A are chemically modified by acylation with fatty acids chlorides. This treatment renders these proteins hydrophobic so that they can combine with the phospholipid membrane of liposomes. This report deals with the incorporation of modified enzymes into liposomes prepared by different methods.