Preparation of monospecific anti-Salmonella lipopolysaccharide antibodies by affinity chromatography

The use of immunoadsorbent obtained by coupling aminohexyl-sepharose 4B with Salmonella lipopolysaccharide (LPS) by means of benzoquinone enabled us to obtain anti-O monospecific immune sera which can be used for a quick serological identification of some species of Salmonella in the course of a diagnosis. In this paper we describe a method for binding the LPS extracted from S. typhi-murium with aminohexyl-sepharose 4B, insoluble matrix as well as the preparation of monospecific anti-O5 antibodies from plurispecific anti-S. haifa rabbit immune sera. This separation of anti-O monospecific antibodies by affinity chromatography, avoids the repeated and often tedious adsorption of anti-Salmonella immune sera by the whole corresponding bacteria. Such immunoabsorbents can be used several times without appreciable loss of their affinity properties.