Concanavalin A binding sites on the luminal surface of ependymal cells of third ventricle

Detection of the Concanavalin A binding sites within the luminal surface of ependymal cells of the third ventricle has been accomplished using perfusion techniques as a way of exposing the cells to reaction solutions. Parameters for this procedure were established. It was found that electron dense reaction products form a layer ranging from 25--40 nm in thickness on the luminal surface of investigated cells. The microvilli exhibited a slightly thiner layer, the reaction products formed small patches on cilia.