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Sex differences in hepatic oestrogen-binding proteins

C Thompson, W Powell-Jones, G W Lucier

    The Biochemical Journal
    |January 15, 1981
    PubMed
    Summary
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    Sex differences in liver cytosol oestrogen-binding proteins emerge post-neonatally, influenced by the hypothalamic-pituitary axis and early androgen exposure, affecting multiple protein peaks.

    Area of Science:

    • Endocrinology
    • Molecular Biology
    • Reproductive Biology

    Background:

    • Oestrogen-binding proteins in hepatic cytosol exhibit sex-specific patterns.
    • Understanding these differences is crucial for comprehending sex-based physiological variations.

    Purpose of the Study:

    • To characterize sex differences in hepatic oestrogen-binding proteins.
    • To investigate the developmental and hormonal influences on these protein profiles.

    Main Methods:

    • Gel-filtration chromatography (Sephadex G-75) of hepatic cytosol from male and female rats.
    • Analysis of [(3)H]oestradiol binding to identify and quantify oestrogen-binding protein species.
    • Studies included immature animals, gonadectomized adults, and hypophysectomized adults.

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    Main Results:

    • Four oestrogen-binding protein peaks (I, II, IV, V) were common to both sexes; peak I (oestrogen receptor) showed equivalent binding.
    • Peaks II, IV, and V had higher binding in males; a male-specific peak (III) was identified.
    • Neonatal castration partially feminized the male profile, while hypophysectomy reduced sex differences, suggesting hypothalamic-pituitary involvement and neonatal imprinting.

    Conclusions:

    • Hepatic cytosol contains multiple oestrogen-binding proteins with distinct sex-specific profiles.
    • The development of these sex differences appears to be imprinted neonatally via the hypothalamic-pituitary axis, requiring androgen exposure.