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Improved automated optical somatic cell counting method for raw milk: collaborative study

W N Kelley

    Journal - Association of Official Analytical Chemists
    |November 1, 1978
    PubMed
    Summary
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    The automated optical somatic cell counting procedure (OSCC II) offers improved precision and accuracy over the direct microscopic somatic cell counting method (DMSCC) for raw milk analysis. This automated method is now official.

    Area of Science:

    • Dairy Science
    • Analytical Chemistry
    • Food Safety

    Background:

    • Somatic cell counts (SCC) are critical indicators of raw milk quality and udder health.
    • Traditional direct microscopic somatic cell counting (DMSCC) is labor-intensive and can be subjective.
    • An improved automated optical somatic cell counting procedure (OSCC II) was developed to address these limitations.

    Purpose of the Study:

    • To collaboratively evaluate the performance of the improved automated optical somatic cell counting procedure (OSCC II).
    • To compare the accuracy and precision of OSCC II against the standard direct microscopic somatic cell counting method (DMSCC).
    • To assess the impact of different sampling rates on OSCC II performance.

    Main Methods:

    • A collaborative study involving five participants analyzed 48 raw milk samples in duplicate.

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  • Samples were processed using an Auto-Analyzer system for automated dilution, clarification, and cell counting via OSCC II.
  • Results were compared against DMSCC counts obtained by three independent analysts.

    • Main Results:

    • OSCC II demonstrated significantly higher precision than DMSCC, with a standard deviation of 0.0434 compared to 0.0825 for DMSCC.
    • The accuracy of OSCC II was comparable to DMSCC.
    • Increasing the sampling rate for OSCC II from 80/hr to 120/hr slightly reduced precision but minimally impacted accuracy.

    Conclusions:

    • The OSCC II procedure is a highly precise and accurate method for somatic cell counting in raw milk.
    • OSCC II offers a significant improvement in efficiency and consistency over DMSCC.
    • The OSCC II method has been officially adopted for raw milk analysis.