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Antibacterial peptide from normal rabbit serum. 2. Compositional microanalysis

S F Carroll, R J Martinez

    Biochemistry
    |October 13, 1981
    PubMed
    Summary
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    Researchers analyzed a rabbit serum bactericide (PC-III) using microanalytical techniques. The study determined PC-III is a single peptide with a unique amino acid composition, including a modified residue.

    Area of Science:

    • Biochemistry
    • Immunology
    • Microbiology

    Background:

    • Normal rabbit serum contains a primary bactericide (PC-III) with antimicrobial properties.
    • Understanding the molecular composition of PC-III is crucial for elucidating its mechanism of action.

    Purpose of the Study:

    • To determine the precise composition and homogeneity of the primary bactericide (PC-III).
    • To characterize the amino acid profile and identify any modified residues within PC-III.

    Main Methods:

    • Microanalytical techniques were employed to examine PC-III.
    • Dansylated acid hydrolysates of PC-III underwent two-dimensional thin-layer chromatography on polyamide layers.
    • Quantitation of dansyl amino acids was performed using fluorescence detection after elution.

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    Main Results:

    • The analysis revealed PC-III is a single peptide with a molecular weight of 2000 daltons, comprising 17 amino acid residues.
    • The peptide composition includes 24% basic and 35% nonpolar amino acids.
    • A modified basic amino acid, tentatively identified as N epsilon-methyllysine, was detected.

    Conclusions:

    • PC-III is a homogeneous peptide bactericide with a defined amino acid composition.
    • The presence of N epsilon-methyllysine suggests a unique structural feature contributing to its activity.
    • Minor carbohydrate and lipid contaminants can be removed without compromising the bactericidal activity.