Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hypertonic cryohemolysis and the cytoskeletal system

F A Green, C Y Jung, J Cuppoletti

    Biochimica Et Biophysica Acta
    |November 6, 1981
    PubMed
    Summary

    Hypertonic cryohemolysis, the bursting of red blood cells in a cold, salty environment, is linked to the spectrin-actin cytoskeleton. This study reveals the critical role of this cellular framework in the phenomenon.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Pictorial review: A Step-by-step Guide to Coronary CT Angiography with Photon-Counting Detector CT.

    The British journal of radiology·2026
    Same author

    Association between Fiber Intake and Risk of Incident Chronic Kidney Disease: The UK Biobank Study.

    The journal of nutrition, health & aging·2023
    Same author

    Cranioplasty: A Multidisciplinary Approach.

    Frontiers in surgery·2022
    Same author

    Full-step Class II Correction Using a Modified C-palatal Plate for Total Arch Distalization in an Adolescent.

    The Journal of clinical pediatric dentistry·2018
    Same author

    Permeability of bimolecular membranes made from lipid extracts of human red cell ghosts to sugars.

    The Journal of membrane biology·2013
    Same author

    Modulation of the absorption coefficient at 1.3 μm in Ge/SiGe multiple quantum well heterostructures on silicon.

    Optics letters·2011

    Area of Science:

    • Cell Biology
    • Membrane Biophysics
    • Erythrocyte Physiology

    Background:

    • Hypertonic cryohemolysis involves red blood cell lysis in a hypertonic environment upon cooling.
    • This phenomenon is temperature-dependent and can be inhibited by agents that disrupt concentration gradients.
    • The molecular mechanisms underlying hypertonic cryohemolysis remain unclear.

    Purpose of the Study:

    • To elucidate the molecular basis of hypertonic cryohemolysis.
    • To investigate the involvement of the erythrocyte membrane's cytoskeletal framework.

    Main Methods:

    • Radiation-induced ablation to determine the required macromolecular complex size.
    • Selective cross-linking of spectrin and actin using diamide.
    • Polyacrylamide gel electrophoresis to analyze protein aggregation.

    Main Results:

    • An intact macromolecular complex of approximately 16,000,000 daltons is necessary for hypertonic cryohemolysis.
    • Diamide treatment suppressed cryohemolysis in a concentration-dependent manner.
    • Diamide induced protein aggregation, including loss of spectrin-actin and associated proteins.

    Conclusions:

    • The spectrin-actin cytoskeletal framework is critically involved in hypertonic cryohemolysis.
    • Interactions between the cytoskeleton and membrane phospholipids may play a key role.

    Related Experiment Videos