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[Enzymatic oxidation of glucose on modified electrodes]
Biokhimiia (Moscow, Russia)
|October 1, 1981
Summary
This study demonstrates that glucose oxidase entrapped with various mediators electrocatalytically oxidizes glucose, achieving maximal currents dependent on enzyme and mediator concentration.
Area of Science:
- Electrochemistry
- Biocatalysis
- Biosensors
Context:
- Development of electrochemical biosensors for glucose detection.
- Utilizing enzymes immobilized on electrode surfaces.
- Exploring mediator compounds to enhance electron transfer in biosensors.
Purpose:
- To investigate the electrocatalytic oxidation of glucose using glucose oxidase immobilized with different mediators.
- To determine the effect of mediator type and enzyme concentration on glucose oxidation current.
- To understand the mediator fashion of electrocatalytic glucose oxidation.
Summary:
- Glucose oxidase (EC 1.1.3.4) was entrapped with mediators like tetracyano-p-quinodimethane and piocyanine on a glass-carbon electrode.
- Electrocatalytic oxidation of glucose was achieved, with maximal currents reaching 67 micro A/cm².
- The current output was influenced by enzyme concentration and the specific mediator used, indicating a mediator-driven process.
Impact:
- Provides insights into designing efficient glucose biosensors.
- Highlights the role of mediators in enhancing enzyme-electrode electron transfer.
- Contributes to the field of electrochemical sensing for biological analytes.