Endotrophic sporulation by the yeast Nadsonia fulvescens

Studies of carbon requirements for sporulation by Nadsonia fulvescens showed an unexpected appearance of spores in the absence of an exogenous carbon source, when the cells were grown on ethanol prior to induction of sporulation, Dextrose-grown cells taken from either the logarithmic or stationary phase required a carbon source (dextrose, ethanol, or glycerol) for sporulation. An inhibitory effect by acetate upon sporulation was demonstrated, and it appears to be due to a repression mechanism.