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Polymorphonuclear leukocyte migration through human amnion membrane

R G Russo, L A Liotta, U Thorgeirsson

    The Journal of Cell Biology
    |November 1, 1981
    PubMed
    Summary
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    A new in vitro model using human amnion membrane allows scientists to study polymorphonuclear leukocyte (PMN) migration through native barriers. This advanced system mimics in vivo inflammation, offering insights into cell movement and potential repair mechanisms.

    Area of Science:

    • Cell Biology
    • Immunology
    • Biomedical Engineering

    Background:

    • Studying polymorphonuclear leukocyte (PMN) migration is crucial for understanding inflammatory responses.
    • Previous in vitro models lack the complexity of native cellular and matrix barriers found in vivo.
    • A need exists for a more physiologically relevant model to investigate PMN transmigration.

    Purpose of the Study:

    • To develop and validate a novel in vitro model for studying human PMN migration through living native barriers.
    • To analyze the factors influencing PMN migration rate and route using this new model.
    • To provide a platform for future studies on PMN-matrix interactions and inflammatory processes.

    Main Methods:

    • Utilized human amnion membrane, comprising native epithelium, basement membrane, and collagenous stroma, as the barrier.

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  • Developed a two-compartment chamber system to simulate physiological conditions for PMN migration.
  • Employed N-formylmethionyl-leucyl-phenylanlanine (FMLP) as a chemoattractant and transmission electron microscopy for route analysis.

    • Main Results:

    • PMN successfully migrated through the full thickness of the native amnion barrier in response to FMLP.
    • Migration rate was dependent on FMLP concentration and gradient slope, with optimal chemoattraction at 10(-8)M.
    • Transmission electron microscopy revealed PMN infiltration between epithelial cells, through junctions, basement membrane, and collagenous stroma.

    Conclusions:

    • The developed amnion migration system accurately models in vivo inflammatory cell migration through complex native barriers.
    • This model recapitulates key aspects of PMN transmigration, including interaction with epithelium, basement membrane, and stroma.
    • The system offers a valuable tool for investigating the biochemical and morphological mechanisms underlying PMN penetration and potential tissue repair.