Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Improved viscometric assay for cellulase

K Manning

    Journal of Biochemical and Biophysical Methods
    |October 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    A new viscometric assay accurately measures carboxymethylcellulase activity by accounting for kinetic energy and non-Newtonian behavior. This method corrects for product inhibition, enabling precise initial velocity determination in enzymatic reactions.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Canadian Surgery Forum: Abstracts of presentations to the Annual Meetings of the Canadian Association of Bariatric Physicians and Surgeons, Canadian Association of General Surgeons, Canadian Association of Thoracic Surgeons, Canadian Hepato-Pancreato-Biliary Association, Canadian Society of Surgical Oncology, Canadian Society of Colon and Rectal Surgeons, Vancouver, BC, Sept. 17-21, 2013.

    Canadian journal of surgery. Journal canadien de chirurgie·2025
    Same author

    Clinical course, management and outcomes of COVID-19 in HIV-infected renal transplant recipients: A case series.

    South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2024
    Same author

    The state of paediatric deceased-organ donation at a South African tertiary public- sector hospital: A 14-year analysis.

    South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2024
    Same author

    Kidney transplant utilising donors after circulatory death: The first report from the African continent.

    South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2024
    Same author

    The state of kidney replacement therapy in Eastern Cape Province, South Africa: A call to action.

    South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2023
    Same author

    One-year mortality after hospital admission as an indicator of palliative care need: A retrospective cohort study.

    South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2021
    Same journal

    Proceedings of the 12th International Symposium Of Chemistry, Miercurea Ciuc (Csíkszereda), Romania, October 5-8, 2006; and proceedings of the 7th International Symposium and Summer School on Bioanalysis, Pécs, Hungary, June 10-17, 2007.

    Journal of biochemical and biophysical methods·2008
    Same journal

    12th International Symposium Of Chemistry, Miercurea Ciuc (Csíkszereda), Romania, 5th to 8th of October, 2006. 7th International Symposium and Summer School on Bioanalysis, Pécs, Hungary, 10th to 17th of June, 2007.

    Journal of biochemical and biophysical methods·2008
    Same journal

    Optimized HPLC method for tramadol and O-desmethyl tramadol determination in human plasma.

    Journal of biochemical and biophysical methods·2008
    Same journal

    The alternating current polarographic behavior and determination of lansoprazole and omeprazole in dosage forms and biological fluids.

    Journal of biochemical and biophysical methods·2008
    Same journal

    Continuous-flow complete-mixing system for assessing the effects of environmental factors on colony-level coral metabolism.

    Journal of biochemical and biophysical methods·2008
    Same journal

    Utilization of fluorescence tracer in hyperinsulinemic-euglycemic clamp test in mice.

    Journal of biochemical and biophysical methods·2008
    See all related articles

    Area of Science:

    • Biochemistry
    • Enzymology
    • Polymer Science

    Background:

    • Carboxymethylcellulose (CMC) is a widely used polymer in various industries.
    • Accurate determination of carboxymethylcellulase (CMCase) activity is crucial for understanding enzyme kinetics and optimizing industrial processes.
    • Existing methods for CMCase activity assay may not fully account for complex solution behaviors or product inhibition.

    Purpose of the Study:

    • To develop and validate a novel viscometric assay for quantifying carboxymethylcellulase activity.
    • To address limitations of existing assays by incorporating theoretical corrections for kinetic energy and non-Newtonian fluid dynamics.
    • To establish a reliable method for estimating initial reaction velocities in the presence of product inhibition.

    Main Methods:

    Related Experiment Videos

  • Development of a viscometric assay based on a direct theoretical approach.
  • Inclusion of kinetic energy correction factors.
  • Characterization of non-Newtonian behavior of carboxymethylcellulose solutions.
  • Application of a method to estimate initial reaction velocities, accounting for competitive product inhibition.
  • Validation using direct chemical determination of reducing end groups.

    • Main Results:

    • The developed viscometric assay accurately determines carboxymethylcellulase activity.
    • The assay effectively accounts for kinetic energy and non-Newtonian properties of CMC solutions.
    • A robust method for estimating initial reaction velocities in the presence of product inhibition was successfully implemented.
    • Results were validated through direct chemical analysis of reducing end groups, confirming assay validity.

    Conclusions:

    • The novel viscometric assay provides a theoretically sound and accurate method for measuring carboxymethylcellulase activity.
    • The assay's ability to correct for product inhibition and non-Newtonian behavior enhances its reliability for kinetic studies.
    • This method offers a valuable tool for researchers and industrial applications involving carboxymethylcellulase enzymes.