Related Experiment Video
Updated: Apr 3, 2026

Fabrication and Implantation of Miniature Dual-element Strain Gages for Measuring In Vivo Gastrointestinal Contractions in Rodents.
Published on: September 18, 2014
Studies of a gastric fundic mucosal factor which affects absorption from the antrum and jejunum in the rat
Abstract:
1. A rat bio-assay has been developed in which the tracer ion technetium 99 m (99mTc) was used to monitor absorption from both the antrum and the jejunum of the rat. 2. Evidence has been obtained for the release of a humoral substance from the rat gastric fundus after alkalinization of the mucosa at pH 8, and this fundic factor has been shown to reduce the absorption of the tracer ion 99mTc from both the lumen of the antrum and of the jejunum in the rat. 3. Intravenous infusion of a neutral extract of porcine fundic mucosa, distension of the rat fundus with air or exposure of the fundic mucosa to alkaline buffer all decreased absorption of the tracer ion virtually to the same extent from the lumen of the antrum (P less than 0.001) and to a lesser extent from the lumen of the jejunum (P less than 0.05). This effect does not appear to be due to histamine. 4. Preliminary biochemical data have indicted that the biological activity in the mucosal extract is protein in nature and has a molecular weight of less than 10,000.
Related Concept Videos
Drug Absorption: Factors Affecting GI Absorption
Pathophysiology of Peptic Ulcer Disease: Injurious Factors
In the antrum region, G cells secrete the gastrin hormone that binds to gastrin-cholecystokinin-B (CCK2) receptors on parietal and enterochromaffin-like (ECL) cells in the fundic glands. Simultaneously, the vagus nerve releases acetylcholine, which binds...
Factors Influencing Drug Absorption: Anatomical Parameters
Factors Influencing Drug Absorption: Disease States and Pharmacology
Substances such as alcohol and specific drugs, including antineoplastics, can also negatively impact drug absorption. For instance,...
Methods for Studying Drug Absorption: In vitro
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
Methods for Studying Drug Absorption: In situ
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...

