The effect of prolonged propranolol administration on myocardial transmural capillary density in young rabbits

1. As a sequel to previous evidence that prolonged beta-adrenoceptor blockade in young rabbits led to a relative increase in the capillarity of ventricular muscle, an attempt has been made to localize and quantify the capillary density from epicardium to endocardium in 12-week old rabbits treated for 6 weeks with propranolol (3 mg kg-1) S.C. twice daily and in litter-mates treated with saline (controls). 2. Full-thickness 0.5 micrometers sections of the left ventricular wall were point-counted in 200 micrometers steps. In the controls the capillary 'volume density' decreased progressively below the epicardial surface from a value of 9% to reach a minimum of 7.1% at a depth of 2.5 mm. Capillary density then increased again towards the endocardium, reaching a maximum of 11.5% in the trabeculae carnae (depth 3.5 mm). 3. In the treated animals the capillary density was increased in the mid wall at 2.3 and 2.5 mm, but significantly decreased in the trabeculae at 3.7 mm (P less than 0.05). 4. A more detailed analysis was undertaken at depths of 0.7 (Epi), 2.3 (Endo) and 3.5 mm (Trab), comprising estimates of capillary number, capillary diameter and intercapillary distance, via the stereological parameters of mean intercept length and mean free distance. 5. In the controls the order was Epi greater than Trab greater than Endo for number, Trab greater than Endo greater than Epi for diameter and Endo greater than Epi greater than Trab for intercapillary distance. 6. In the treated rabbits the mean intercapillary distance was significantly reduced in the epicardial (by 17.5%, P less than 0.05) and endocardial zones (by 32%, P less than 0.005), but was not significantly altered (+3.7%, P greater than 0.05) in the trabeculae. 7. The non-uniform distribution of capillaries in the controls illustrates that detection of changes in transmural capillary density requires that the depth of samples be measured from the epicardial surface and not, owing to the presence of trabeculae carnae, from the endocardium. 8. If similar treatment effects were to occur in man, they would suggest that prolonged beta-blockade could induce an increase in capillary density which would render the ventricular mid wall less susceptible to ischaemia.