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[Electron microscopic study of chick embryo myogenic cells in a suspension culture]
Abstract:
Cell aggregates arising in the suspension culture of the chick embryo myogenic cells were studied by means of electron microscope. The small aggregates are formed by mononuclear myoblasts. The large aggregates are characterized by the tissular organization with the orderly distribution of different cell types. The periphery of such aggregates is occupied by polynuclear myosimplasts in different parts of which individual characters of muscle fiber are expressed to different extent. The cells similar with fibroblasts, chondroblasts, chondrocytes and even fatty cells, as well as collagenous extracellular matrix are situated in the centre of large aggregates. The possibility of origin of different cell types from myoblasts under the specific conditions of suspension culture is discussed.