Sequential [125I]-o-iodohippurate renograms from rabbit kidneys after temporary renal ischemia

Serial renography with [125I]-o-iodohippurate was performed in 42 rabbits after temporary clamping of the renal artery for 1--3 hr and contralateral nephrectomy. Renograms were done before and after warm ischemia and followed 2, 4, 6, and 14 days after restoration of circulation. Correlation was found between duration of ischemia and severity of postischemic renographic changes. Most sensitive was the third phase, which showed distortion after 1 hr of ischemia; the second phase decreased after 2 1/2 hours of ischemia. The profound changes seen on the second day were shown to be due to further damage of the tubular cell system after restored flow. In vitro [125I]-o-iodohippurate uptake in cortex slices showed a significant decrease after warm ischemia damage and 48 hours restored flow. The second phase recovered more rapidly than the excretory function.