Establishment of pigmented melanocyte culture strain from Harding-Passey melanoma

A cultured cell strain of melanotic melanoma was established from Harding-Passey mouse melanoma. The established cells proliferated as a monolayer culture and actively produced melanin pigment. Under an electron microscope, these cells showed the typical characteristic features of the melanocyte. When a cell suspension was inoculated into Swiss mice, they developed typical melanocytic tumors which closely resembled the original melanomas.