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Selective migration of 'null' cells towards a thymus factor in vitro
Thymus
|November 1, 1981
Summary
Researchers depleted specific immune cells (theta+, Ig+) from mouse fetal liver and bone marrow using thymus supernatant. This method yields large numbers of viable cells for in vivo testing.
Area of Science:
- Immunology
- Cell Biology
Background:
- Immune cell populations in fetal liver and adult bone marrow are critical for immunological studies.
- Isolating specific immune cell subsets, such as theta-positive (theta+) and immunoglobulin-positive (Ig+) cells, is essential for various research applications.
Purpose of the Study:
- To develop an efficient method for depleting theta+ and Ig+ cells from mouse fetal liver and bone marrow.
- To obtain large quantities of viable, non-theta+ and non-Ig+ cells for subsequent in vivo research.
Main Methods:
- Utilizing in vitro migration of cells towards a thymus supernatant attractant.
- Employing a porous membrane to facilitate cell separation based on chemotaxis.
- Using multiple migration wells to maximize cell yield.
Main Results:
- Successful depletion of theta+ and Ig+ cells from mouse fetal liver, nude adult bone marrow, and normal adult bone marrow.
- The attracted cells, now depleted of theta+ and Ig+ populations, were found to be free in suspension and viable.
- The method demonstrated the potential to obtain a large number of these depleted cells.
Conclusions:
- In vitro migration towards thymus supernatant is an effective technique for depleting specific immune cell populations.
- This method provides a scalable approach to isolate viable immune cells for in vivo studies.